548 Purinergic molecules in murine bone marrow-derived mast cells

نویسندگان

چکیده

In the skin, adenosine triphosphate (ATP) is released from various types of cells by environmental stimuli via nonlytic mechanisms, cell damage, or acute death. Because ATP a potent inducer skin inflammation, it has to be promptly hydrolyzed for achieve homeostasis. Of note, activates mast (MCs) through P2X7R, leading enhanced inflammation. However, MC involvement in impairment inflammation hydrolysis remains largely unknown. Thus, we sought determine expression ATP-hydrolyzing molecules MCs. Bone marrow recovered female B6 mice were cultured presence stem factor and IL-3 5 weeks, resulting differentiation into CD45+FcεRI+c-kit+ bone marrow-derived (BMMCs). A culture BMMCs with 1 mM ATP-γ-S, non-metabolizable analogue, μM ionomycin 60 min 10 min, respectively, induced their degranulation. PBS-treated steady-state strongly expressed Entpd-1 (CD39) Entpd-3, weakly Enpp-1 CD73, but not Entpd-2, Entpd-8, Enpp-2, Enpp-3, ALP. While upregulated (CD39), expressions approximately four times, ATP-γ-S only times. These data suggest that MCs might participate Entpd-3. single-cell suspension back clearly express at protein levels. Steady-state potently hydrolyze exogenous ATP. Moreover, ionomycin-stimulated trended towards increase activity. Collectively, murine functional such as thereby contributing

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ژورنال

عنوان ژورنال: Journal of Investigative Dermatology

سال: 2022

ISSN: ['1523-1747', '0022-202X']

DOI: https://doi.org/10.1016/j.jid.2022.05.558